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(3) With reduced angular aperture, the unit diffraction spots comprising the image become larger, causing lines and edges to become thicker and cover a greater number of photoreceptor cells on the retina, thus making the demarcations appear darker and easier to see Thus, the benefits of improved contrast and visibility might lead us to select a slightly stopped-down condenser aperture, even though spatial resolution has been compromised slightly in the process If the condenser aperture is closed down too far, however, the image loses significant spatial resolution and the dark diffraction edges around objects become objectionable Thus, the principles of image formation must be understood if the microscope is to be used properly A wide aperture allows maximal spatial resolution, but decreases contrast, while a smaller, constricted aperture improves visibility and contrast, but decreases spatial resolution For all specimens, the ideal aperture location defines a balance between resolution and contrast.

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The supporting electrolytes influence polarographic and voltammetric measurements in the manners as described below: a) The cation or anion of the supporting electrolyte reacts at the electrode: At a mercury electrode, the negative side of the potential window is narrowed by an alkali metal ion which is reduced to its amalgam, while the positive side by a halide ion which facilitates the anodic dissolution of mercury. At a platinum or carbon electrode, a halide ion is anodically oxidized and narrows the positive side of the potential window. These influences of anions are observed also in water, but more marked in aprotic solvents. With tetraalkylammonium salts of ClO , BF , PF and 4 4 6 CF3SO , these influences can be avoided. 3 b) The cation or anion of the supporting electrolyte interacts with the electroactive substances: A typical example is when a halide ion forms a complex with an electroactive metal ion and shifts the reduction potential of the metal ion to negative direction. Sometimes a halide ion forms a precipitate with an electroactive metal ion. These influences occur also in water but are more pronounced in aprotic solvents.

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A useful guideline for beginners is to stop down the condenser aperture to about 70% of the maximum aperture diameter, but this is not a rigid rule If we view specimens such as a diffraction grating, a diatom, or a section of striated muscle, stopping down the diaphragm to improve contrast might suddenly obliterate periodic specimen details, because the angular aperture is too small to allow diffracted light to be collected and focused in the image plane In such a situation, the aperture should be reopened to whatever position gives adequate resolution of specimen detail and acceptable overall image visibility and contrast..

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